This claim is true. Donald Trump has consistently spoken out about his distaste for transgender athletes in women's sports. An article from AP News quotes Donald Trump, “With this executive order, the war on women’s sports is over,”. He is referring to the executive order “Keeping Men Out of Women’s Sports” that he signed at the beginning of February. AP News tells us, “gives federal agencies wide latitude to ensure entities that receive federal funding abide by Title IX in alignment with the Trump administration’s view, which interprets “sex” as the gender someone was assigned at birth.”.
Further looking into this order, The official website of the White House tells as a presidential action, the order was signed by President Donald Trump. Reading through the fine print of this order we can further confirm this statement of his plans of banning transgender athletes in women's sports. The order specifically states, “It shall also be the policy of the United States to oppose male competitive participation in women’s sports more broadly, as a matter of safety, fairness, dignity, and truth.”. For context, this order refers to transgender athletes as “male competitors” and “eligibility for participation in women’s sporting events is determined according to sex and not gender identity or testosterone reduction”. This direct source from the White House tells us that Trump is planning and working towards banning transgender athletes from womens sports.
https://www.whitehouse.gov/presidential-actions/2025/02/keeping-men-out-of-womens-sports/
https://apnews.com/article/donald-trump-transgender-athletes-3606411fc12efffec95a893351624e1b